Advances in Research and Applications - download pdf or read online

By G.D. Aurbach and Donald B. McCormick (Eds.)

ISBN-10: 0080866441

ISBN-13: 9780080866444

ISBN-10: 012709847X

ISBN-13: 9780127098470

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Heparan sulfate and chondroitin sulfate also inhibited formation of the ternary complex, but were much less effective than heparin. Baxter proposed that proteoglycans at the surface of capillary endothelial cells might facilitate the dissociation of the ALS from the ternary complex, releasing 1GF:IGFBP-3 complexes, and providing a potential mechanism for circulating IGFs to enter the extravascular space. 6. Regulation of ALS in Human Plasma Antiserum was raised to purified human ALS and a radioimmunoassay established (Baxter, 1990b).

Mature rat and human IGFBP-6 contain 201 and 216 amino acids, respectively, and have molecular masses of 21,461 and 22,847 Da. The difference from the apparent molecular mass’of human IGFBP-6 on ligand blot (28-34 kDa) presumably reflects the presence of 0-linked oligosaccharides. The NH,-terminal region of IGFBP-6 differs from that of IGFBPs 15. The amino-terminal “GCGCC” sequence conserved in IGFBPs 1-5 is modified in rat (“GCAET) and human (“GCAEA”) IGFBP-6. l In addition, in rat IGFBP-6, the region between the second and the fifth cysteines (including the third and fourth cysteines) is not conserved; these cysteines are retained in human IGFBP-6.

Human. , 1989b). , 1989). Roghani et al. (1991) confirmed that the NH,-terminal amino acid sequence of the 34-kDa protein corresponded to the amino acid sequence of IGFBP-2 deduced from the nucleotide sequence of cDNA clones. , 1989a). The smear was not seen using IGF-I tracer (Yang and Rechler, 19921, suggesting that it represented a second IGFBP with preferential affinity for IGF-11. Roghani et al. (1991) separated a 30- to 32-kDa IGFBP from the more hydrophobic 34-kDa IGFBP-2 using reverse-phase HPLC and 34 MAVHEW M.

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Advances in Research and Applications by G.D. Aurbach and Donald B. McCormick (Eds.)


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