By Wayne W Umbreit
Meant for researchers in utilized microbiology and environmental engineers, this e-book covers such issues as environmental review of biotechnological techniques and microbial changes of haloaromatic and haloaliphatic compounds.
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Extra info for Advances in Applied Microbiology, Vol. 3
Tube may become plugged before drying is completed. The secondary trap, which effectively prevented contamination of the pump oil with water, consisted of a loop of tygon tubing submerged in the same ethanol-dry-ice bath as the flask. This apparatus, though not as ‘efficient as the more elaborate systems, is effective and can truly be said to be a do-it-yourself apparatus. Care must be exercised to select glassware that will withstand vacuum. Pyrex spherical or cylindrical flasks should always be used, although the shape is not too important for small condensers.
Implying, at least, that they considered these factors to have some influence on survival. Although a marked difference between cells grown on agar surface and submerged liquid culture might be expected this factor has scarcely been studied. Solid cultures, obtained from agar slants or plates, have been used generally for aerobic organims by those interested in preserving large collections (Proom and Hemmons, 1949; Rhodes and Fisher, 1950) because it was convenient. , (1958). Verwey (1945) and Verwey and Scheidy (1946) used solid medium because that was the prescribed method for producing Brucella vaccine, Van Drimmelen (1956) is one of the few who have compared the survival rate of organisms grown on solid versus liquid medium.
POOC. - rvr RT Papain digest broth 0 2-3 Months 24-30 Months 96 49-109 100 68 36 References Annear (1958a) Fry and Greaves (1951) 8'2 Weeks 20 Scott (1958) 2 Days 3 Months 0 Months 12 Months 17 16 15 14 Birkhaug (1951) 4°C. 0 6 Months 37 12 B@eand Evjen (1952) 5°C. 5°C. RT RT 3-4 Months 6 Months 1 Month 5 Months 100 5°C. 37°C. 37°C. 5°C. 37°C. 37°C. 0 12 Months 2 Months 12 Months 0 12 Months 2 Months 12 Months 10 3 1 % Sodium glutamate 1 % Sucrose 5°C. 37°C. 5°C. 37°C. 6 Months 6 Months 6 Months 6 Months 10% Sucrose and 1% gelatin - 0 Mycobacterium tuberculosis (BCG) 50% Glucose Milk whey plus 3 % glucose 50 % Glucose 1% Sucrose 1 % Sodium glutamate 25°C.
Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit